Systematic identification of H-2 Kd binding peptides and induction of peptide specific CTL
Identifieur interne : 000343 ( France/Analysis ); précédent : 000342; suivant : 000344Systematic identification of H-2 Kd binding peptides and induction of peptide specific CTL
Auteurs : Randall F. Gill [États-Unis] ; Jean-Pierre Abastado [France] ; Wei-Zen Wei [États-Unis]Source :
- Journal of Immunological Methods [ 0022-1759 ] ; 1994.
English descriptors
- Teeft :
- Abastado, Additional constraints, Aliphatic residue, Allelic motif, Amino acid, Antigen processing, Antigenic, Antigenic peptides, Assay, Binding activity, Binding assay, Binding peptides, Cell lines, Cell receptors, Chain fusion protein, Competition binding assay, Control peptide, Control peptide syipsaeyl, Fusion protein, Gill, Histopaque gradient, Immunization, Immunological methods, Incomplete adjuvant, Last injection, Lmmunological methods, Lymph node cells, Lymphocyte, Lysis, Major histocompatibility, Methods section, Mitomycin, Mitomycin splenocytes, Mmtv, Mmtv peptide, Mmtv peptides, Node, Open circle, Open square, Open triangle, Peptide, Peptide binding, Pool sequencing, Single chain, Splenocytes, Strong binding, Syngeneic splenocytes, Synthetic peptides, Tail base, Target ceils, Target cells, Test peptides, Tumor virus, Unbound peptide, Unpublished observation, Viable cells, Vivo immunization, Vivo immunizations, Weak binding, Week intervals.
Abstract
Abstract: Most peptides with putative MHC I restricted sequence motifs do not bind to the corresponding MHC I nor induce cytolytic T cells. There exist additional constraints which limit peptide binding and immunogenicity. To identify immunogenic peptides in novel protein sequences, it will be necessary to first evaluate peptide binding to MHC I. In this study, a soluble single chain fusion protein SC-Kd was used to evaluate potential Kd binding peptides from the sequences of mouse mammary tumor virus gag and env proteins. A total of 27 peptides were identified which displayed the reported Kd restricted motif. Of the 27 peptides, six demonstrated strong to moderate binding to SC-Kd. The strongest binding peptides expressed tyrosine or phenylalanine at position 2 and leucine at the C-terminus. The capability of MMTV peptides to induce CTL corresponds to their SC-Kd binding activity. Of the six peptides that demonstrated moderate to strong binding, five induced CTL in BLAB/c mice. These peptides induced CTL after 1–3 in vivo immunizations followed by 5 day in vitro stimulation. Furthermore, a single in vitro stimulation of naive lymphocytes with strong-binding G425 was sufficient to induce significant CTL activity. Weak or non-binding peptides did not induce CTL. Therefore, peptide binding to SC-Kd is a predictive indicator of CTL inducing activity.
Url:
DOI: 10.1016/0022-1759(94)90318-2
Affiliations:
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Gill</name></author><author><name sortKey="Abastado, Jean Pierre" sort="Abastado, Jean Pierre" uniqKey="Abastado J" first="Jean-Pierre" last="Abastado">Jean-Pierre Abastado</name></author><author><name sortKey="Wei, Wei Zen" sort="Wei, Wei Zen" uniqKey="Wei W" first="Wei-Zen" last="Wei">Wei-Zen Wei</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:1C58EA4A8BDBEC2FFFCDD485FB881644C3FD8038</idno><date when="1994" year="1994">1994</date><idno type="doi">10.1016/0022-1759(94)90318-2</idno><idno type="url">https://api.istex.fr/ark:/67375/6H6-27H695K8-V/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000917</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000917</idno><idno type="wicri:Area/Istex/Curation">000917</idno><idno type="wicri:Area/Istex/Checkpoint">001947</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001947</idno><idno type="wicri:doubleKey">0022-1759:1994:Gill R:systematic:identification:of</idno><idno type="wicri:Area/Main/Merge">004373</idno><idno type="wicri:Area/Main/Curation">004310</idno><idno type="wicri:Area/Main/Exploration">004310</idno><idno type="wicri:Area/France/Extraction">000343</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Systematic identification of H-2 Kd binding peptides and induction of peptide specific CTL</title><author><name sortKey="Gill, Randall F" sort="Gill, Randall F" uniqKey="Gill R" first="Randall F." last="Gill">Randall F. Gill</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Immunology, Breast Cancer Program, Michigan Cancer Foundation, 110 E. Warren Ave., Detroit, MI 48201</wicri:regionArea><wicri:noRegion>MI 48201</wicri:noRegion></affiliation></author><author><name sortKey="Abastado, Jean Pierre" sort="Abastado, Jean Pierre" uniqKey="Abastado J" first="Jean-Pierre" last="Abastado">Jean-Pierre Abastado</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Department d'Immunologie, Institut Pasteur, Paris</wicri:regionArea><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Wei, Wei Zen" sort="Wei, Wei Zen" uniqKey="Wei W" first="Wei-Zen" last="Wei">Wei-Zen Wei</name><affiliation></affiliation><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Immunology, Breast Cancer Program, Michigan Cancer Foundation, 110 E. Warren Ave., Detroit, MI 48201</wicri:regionArea><wicri:noRegion>MI 48201</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Immunological Methods</title><title level="j" type="abbrev">JIM</title><idno type="ISSN">0022-1759</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1994">1994</date><biblScope unit="volume">176</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="245">245</biblScope><biblScope unit="page" to="253">253</biblScope></imprint><idno type="ISSN">0022-1759</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0022-1759</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Abastado</term><term>Additional constraints</term><term>Aliphatic residue</term><term>Allelic motif</term><term>Amino acid</term><term>Antigen processing</term><term>Antigenic</term><term>Antigenic peptides</term><term>Assay</term><term>Binding activity</term><term>Binding assay</term><term>Binding peptides</term><term>Cell lines</term><term>Cell receptors</term><term>Chain fusion protein</term><term>Competition binding assay</term><term>Control peptide</term><term>Control peptide syipsaeyl</term><term>Fusion protein</term><term>Gill</term><term>Histopaque gradient</term><term>Immunization</term><term>Immunological methods</term><term>Incomplete adjuvant</term><term>Last injection</term><term>Lmmunological methods</term><term>Lymph node cells</term><term>Lymphocyte</term><term>Lysis</term><term>Major histocompatibility</term><term>Methods section</term><term>Mitomycin</term><term>Mitomycin splenocytes</term><term>Mmtv</term><term>Mmtv peptide</term><term>Mmtv peptides</term><term>Node</term><term>Open circle</term><term>Open square</term><term>Open triangle</term><term>Peptide</term><term>Peptide binding</term><term>Pool sequencing</term><term>Single chain</term><term>Splenocytes</term><term>Strong binding</term><term>Syngeneic splenocytes</term><term>Synthetic peptides</term><term>Tail base</term><term>Target ceils</term><term>Target cells</term><term>Test peptides</term><term>Tumor virus</term><term>Unbound peptide</term><term>Unpublished observation</term><term>Viable cells</term><term>Vivo immunization</term><term>Vivo immunizations</term><term>Weak binding</term><term>Week intervals</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Most peptides with putative MHC I restricted sequence motifs do not bind to the corresponding MHC I nor induce cytolytic T cells. There exist additional constraints which limit peptide binding and immunogenicity. To identify immunogenic peptides in novel protein sequences, it will be necessary to first evaluate peptide binding to MHC I. In this study, a soluble single chain fusion protein SC-Kd was used to evaluate potential Kd binding peptides from the sequences of mouse mammary tumor virus gag and env proteins. A total of 27 peptides were identified which displayed the reported Kd restricted motif. Of the 27 peptides, six demonstrated strong to moderate binding to SC-Kd. The strongest binding peptides expressed tyrosine or phenylalanine at position 2 and leucine at the C-terminus. The capability of MMTV peptides to induce CTL corresponds to their SC-Kd binding activity. Of the six peptides that demonstrated moderate to strong binding, five induced CTL in BLAB/c mice. These peptides induced CTL after 1–3 in vivo immunizations followed by 5 day in vitro stimulation. Furthermore, a single in vitro stimulation of naive lymphocytes with strong-binding G425 was sufficient to induce significant CTL activity. Weak or non-binding peptides did not induce CTL. Therefore, peptide binding to SC-Kd is a predictive indicator of CTL inducing activity.</div></front></TEI><affiliations><list><country><li>France</li><li>États-Unis</li></country><region><li>Île-de-France</li></region><settlement><li>Paris</li></settlement></list><tree><country name="États-Unis"><noRegion><name sortKey="Gill, Randall F" sort="Gill, Randall F" uniqKey="Gill R" first="Randall F." last="Gill">Randall F. Gill</name></noRegion><name sortKey="Wei, Wei Zen" sort="Wei, Wei Zen" uniqKey="Wei W" first="Wei-Zen" last="Wei">Wei-Zen Wei</name></country><country name="France"><region name="Île-de-France"><name sortKey="Abastado, Jean Pierre" sort="Abastado, Jean Pierre" uniqKey="Abastado J" first="Jean-Pierre" last="Abastado">Jean-Pierre Abastado</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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